Asymmetric PCR-SSCP: a Useful Tool for Detection of OLA-DRB1(MHC Class Ii) Gene Polymorphism in Slovak Improved Valachian Sheep
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منابع مشابه
Asymmetric PCR-SSCP: a Useful Tool for Detection of OLA-DRB1 (MHC Class II) Gene Polymorphism in Slovak Improved Valachian Sheep
Tkáãiková a. , M. R. , Bhide I . Mikula: Asymmetric PCR-SSCP: a Useful Tool for Detection of OLA-DRB1(MHC class II) Gene Polymorphism in Slovak Improved Valachian Sheep. Acta Vet. Brno 2005, 74: 275-278. Detection of OLA-DBR1 (exon 2) gene polymorphism is presented in the paper. Rapid and inexpensive polymorphism detection method, namely, single stranded conformation polymorphism (SSCP) was ass...
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amide gel electrophoresis (PAGE) on a 180× 160× 1.5-mm 10% polyacrylamide gel. The separated DNA strands were subsequently visualized by staining with either EtdBr or silver (2). Separation of the DNA strands before electrophoresis using streptavidin paramagnetic beads consistently yielded single-stranded products upon SSCP analysis (Figure 1). Treatment of DNA strands with S1 nuclease before S...
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tumor necrosis factor -alpha (tnfα) is a proinflammatory cytokine that plays critical roles in the regulation of immune responses including macrophages activity and apoptosis. tnfα gene is polymorphic and its haplotypes have been associated with resistance or susceptibility to disease. this study was undertaken to identify the polymorphism of tnfα gene in makoei sheep, using polymerase chain re...
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ژورنال
عنوان ژورنال: Acta Veterinaria Brno
سال: 2005
ISSN: 0001-7213,1801-7576
DOI: 10.2754/avb200574020275